Fig. 6

Comparative analysis of ε-poly-L-lysine synthetase (epls) in novel species. A ε-poly-L-lysine gene cluster comparison in Epichloe festucae (fungal producer), Corynebacterium variabile (bacterial producer), and three novel species from this study (Agrococcus phoenicis, Microbacterium canaveralium, Microbacterium jepli) and Leifsonia virtsii (isolated from ISS) show conserved gene cluster architecture. B Protein sequence alignment of ε-poly-L-lysine synthetase enzymes from these organisms exhibits conserved domains, including NRPS adenylation (A), thiolation (T), transmembrane (TM), and C-terminal tandem domains (C1, C2, C3)