Fig. 3
Supplementation with Bv enhanced host nutrition utilization and immunity against LMBV infection. A Experimental strategy: fish were fed a diet supplemented with Bv for 28 days, and 20 fish were randomly selected and sacrificed for tissue sample collection. The remaining fish were injected with LMBV, with 30 fish used for the mortality test and 60 fish that were subjected to phenotypic analysis and tissue sample collection at 7 DPI. B Effects of dietary Bv on growth performance of largemouth bass (feed coefficient, weight gain rate, and specific growth rate). C Effect of dietary supplementation with Bv on TPS, AMS, and LPS in the FG, MG, and HG of largemouth bass. D Effect of dietary supplementation with Bv on LSZ, SOD, and ACP activity in the FG, MG, and HG of largemouth bass. E Cumulative survival rates of fish in the Bv and Ctrl groups following LMBV intraperitoneal injection (abbreviations: Ctrl-i.p and Bv-i.p). The data are representative of two independent experiments. F LMBV mcp copies (Log10) in SP, FG, MG, and HG of fish (n = 6). G Statistical analysis of differential phenotypes of infected fish in the Ctrl group and Bv-fed group. The red section indicates the proportion of severely diseased fish, the yellow section represents the proportion of mildly diseased fish, and the blue section represents the proportion of asymptomatic (non-phenotypic: non) fish. *p < 0.05, **p < 0.01, ***p < 0.001