Fig. 5
From: Interactions with native microbial keystone taxa enhance the biocontrol efficiency of Streptomyces
Transcriptomic and metabolomic analysis of Streptomyces R02 under Paenibacillus cellulositrophicus CSC13 treatment (using supernatant metabolites). a Two-dimensional principal coordinate analysis (PCoA) of Bray-Curtis dissimilarity comparing the transcriptome of Streptomyces R02 with the co-culture of Streptomyces R02 and P. cellulositrophicus CSC13’s metabolites (71% of variance explained, P = 0.005, n = 12). b Bubble plot showing significantly enriched key genes from the KEGG enrichment analysis. Major transcriptional changes were observed in genes associated with pathways of (i) valine, leucine, and isoleucine degradation, (ii) glycolysis/gluconeogenesis, (iii) inositol phosphate metabolism, (iv) phenylalanine metabolism, and (v) geraniol degradation and secondary metabolite biosynthesis, which are highlighted in red. c PCoA illustrating significant differences in metabolite compositions between Streptomyces R02 only, P. cellulositrophicus CSC13 only, and co-culture of Streptomyces R02 and P. cellulositrophicus CSC13’s metabolites (CSC13 + R02) (62% of variance explained, P = 0.001, n = 18). d Bubble plot showing enriched metabolites in the R02 + CSC13 treatment group compared with the R02-only control. The pathway containing the key metabolite, Erythromycin E, is highlighted in red (Lactones). e Mantel test demonstrating significant correlations between the metabolite Erythromycin E and key genes, and the inset graph showed the structure and abundance of Erythromycin E under different conditions