Fig. 4

Phylogenetic diversity of vertebrate- and invertebrate-associated viruses. Phylogenetic trees were estimated based on amino acid sequences of the RdRp protein for RNA viruses and the replicase protein for DNA viruses. Phylogenetic inference was performed using the maximum likelihood (ML) method with 1000 bootstrap replicates. Branch lengths are indicated by the scale bar. Viruses identified in this study are color-marked according to their hosts, with blue, red, and yellow representing shrews, moles, and hedgehogs, respectively. Novel viruses discovered in this study are labeled with black rectangles, while viruses of known species are labeled with black circles. Human pathogenic viruses are indicated with black figures, and spillover-risk viruses are marked with yellow polygons. See also Fig. S1. Abbreviations of locations and host species can be seen in Table S2