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Fig. 1 | Microbiome

Fig. 1

From: Initial gut microbiota composition is a determining factor in the promotion of colorectal cancer by oral iron supplementation: evidence from a murine model

Fig. 1

Dietary iron supplementation promotes colorectal carcinogenesis in ApcMin/+ mice transplanted with fecal microbiota (FMT) from patients with colorectal cancer (FMT-CRC) but not from healthy controls (FMT-HC) or murine donors. A Experimental design. B Iron concentration in the liver. Grey bars represent normal range for C57BL/6 WT mice. C Colorectal tumor counts and D tumor size. E Hematoxylin and eosin staining for determination of tumor grade. F Immunohistochemical staining for Ki- 67 quantification. Each symbol represents one mouse, FMT-mice (n = 13 (50 ppm); n = 13 (500 ppm)), FMT-HC (n = 24 (50 ppm); n = 26 (500 ppm)) and FMT-CRC (n = 25 (50 ppm); n = 27 (500 ppm)): 3 mice per donor, 10 donors per group. Bars are means ± SEM. P values were obtained using student t-test for FMT-mice. For mice transplanted with human fecal samples (FMT-HC and FMT-CRC) the generalized estimating equations (GEE) to correct for covariance structure of mice from a same donor (n = 3 mice/donor) was used. *P < 0.05, **P < 0.01, ***P < 0.001, n.s.: non-significant

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Microbiome

ISSN: 2049-2618

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