Fig. 5
Biometric assessment of Ae. albopictus adult mosquitoes. A Male/female ratio obtained per each plate well for conventional, axenic, and gnotobiotic (associated with single yeast species) mosquitoes. Since all gnotobiotic larvae (10 different yeast species) could not be generated at the same time, 2 distinct experiments including axenic, conventional, and 5 gnotobiotic larvae were performed. For each experiment, a total of 180 first-instar larvae were monitored for each infection status (conventional, axenic, and gnotobiotic larvae). B Female wing lengths. C Male wing lengths. Points represent wing lengths of individual mosquitoes (n = 20 females and 20 males per infection status). Median wing length and interquartile ranges of all individuals are signified by the black dot and bars, respectively. Abbreviations (Uri +) and (Uri −) indicate whether yeasts are able to secrete or not uricase, respectively. The average riboflavin production by yeast cells (10−6 ng.cell−1) is also provided for each species. Statistically significant differences between groups were identified with Tukey post hoc tests, and columns labelled with different letters are significantly different with a p-value < 0.05. ns, not significant