Fig. 4

Effects of FXR on transcriptional controlling SIRT1 mRNA in LKB1 deacetylation. A Acetylation associated signals were enriched in pigs by PRICUST2. B Acetylation IP for measuring the intensity of LKB1 after FXR activation in the inflammation, showed on the left side of the membrane. The right side showed INPUT. C SIRT1 is significantly enriched in human study, was download and analyzed from GSE14842 (n = 4–5). D, E Relative protein expression of SIRT1 after FXR activation in the inflammation (n = 3). F, G Relative gene level of SIRT1 after FXR activation in the inflammation (F) and in the case of the use of different concentrations of FXR activator (G) (n = 3). H, I Relative protein expression of SIRT1 after treating with FXR siRNA (n = 3). J, K Relative protein expression of SIRT1, LKB1, FXR, and BAX after treating with SIRT1 siRNA (n = 3). L FXR and Sirt1 DNA promoter binding enrichment was detected by ChIP PCR (n = 3). The differences between groups were compared using either student’s t-test or two-way analysis of variance (ANOVA) followed by Tukey’s test for multiple comparisons, and statistical significance was considered at P < 0.05. Data were represented as mean ± SEM, and exhibited *P < 0.05, **P < 0.01, ***P < 0.001