Fig. 1

UGT1A4 mediated CDCA-3β-glucuronide excessed induces BA disorder diarrhea. A, B Secondary bile acids concentration and total bile acid concentration in the colon (n = 8). C, D Proportion of tauro-conjugated bile acids and glycol-conjugated bile acids. E PCOA plot of bile acids on Bray-Curtis dissimilarity matrices (n = 8). F, G Relative protein expression of FXR, BCL2, BAX, and NFκB-P65. H, I Histomorphology and its score. Scale bars, 100 μm. J Bile acid metabolites and enzymes in CDCA metabolism pathway. K Volcano analysis enriched CDCA-3β-glucuronide in the colon. L Content of CDCA-3β-glucuronide (n = 8). M Content of UGT1A4 enzyme (n = 8). N, O, P Correlation between UGT1A4 content and total bile acids level, FXR level, and BAX level. The differences between groups were compared using either student’s t-test or two-way analysis of variance (ANOVA) followed by Tukey’s test for multiple comparisons, and statistical significance was considered at P < 0.05. Data were represented as mean ± SEM, and exhibited *P < 0.05, **P < 0.01, ***P < 0.001. Correlation played on spearman two-tailed test in 99% confidence interval