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Fig. 2 | Microbiome

Fig. 2

From: New insights into the combined effects of aflatoxin B1 and Eimeria ovinoidalis on uterine function by disrupting the gut–blood–reproductive axis in sheep

Fig. 2

The intestinal barrier of sheep is compromised by AFB1 and coccidia treatment. a Histological examination of colon tissue via H&E staining (200×, 400×; n = 4). Red arrows indicate intestinal epithelial cells, yellow arrows denote inflammatory cells, blue arrows represent bleeding points, and green arrows represent coccidia oocysts. b Statistics on crypt depth (n = 6). cf Expression levels of intestinal barrier-related genes Claudin1, Claudin4, Occludin, and ZO-1 (n = 3). Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001 (AFB1, E.o, and AFB1+E.o vs Con); or #p < 0.05, ##p < 0.01, and ###p < 0.001 (AFB1+E.o vs AFB1 and E.o)

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