Fig. 2

The structure of the Cannabis seed microbiome. A The heat tree shows the relative abundance (node colour) and the number of taxa (node size) of the identified seed endophytes on different taxonomic levels. B Dimensionality reduction of the amplicon sequencing variants (ASVs) using UMAP shows bacterial community composition based on a Bray–Curtis dissimilarity matrix, with significant differences between genotypes and domestication grades (P ≤ 0.001). The inset plot shows the separation distances between samples in the different domestication grades. Colours indicate different domestication grades and shape different chemotypes. C Mean relative abundance of the highly abundant (> 5%) members of the microbiome inhabiting seeds of different genotypes on genus level. Different genera are grouped according to bacterial classes using the same colour hue. Blue corresponds to Gammaproteobacteria, red to Bacilli, violet to Actinobacteria, yellow to Alphaproteobacteria, and pink to Bacteroidia, and the remaining genera are displayed in grey as Other. Genotypes with THC content > 0.3% are marked with *