Fig. 5

Biosynthetic gene cluster abundance differs with age. A The plot indicates reads from nasal (blue) and oral (red) infant and adult microbiomes that mapped to BGCs (aryl polyene, NRPS, PKS, RiPP, siderophore, terpene, and hybrid clusters) identified from upper respiratory bacteria (eHOMD habitat listed as “nasal,” “nasal, oral,” or “oral”). B Reads from nasal (blue) and oral (red) infant and adult microbiomes mapping to BGCs from upper respiratory bacteria, grouped by cluster type. All metagenomes were subsampled to 160 K reads. Fifty-one infant nasal, 22 adult nasal, and 1 adult oral metagenomes were not included in analysis due to low sequencing depth. Normalized reads were pseudoaligned onto an in-house BGC library. NRPS, non-ribosomal peptide synthases; PKS, polyketide synthases; RiPP, ribosomally synthesized and post-translationally modified peptides. Significance value *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 Yuen Welch’s T-test with 0.001 trim