Fig. 5

Comparison of different library strategies for fecalvirome communities. A Principal coordinates analysis (PCoA) plots of Bray–Curtis distance matrices. PCoA was used to plot the beta diversity of viral-associated communities using the bray matrix. Different colors indicate different library preparation methods. For each axis, in square brackets, the percentage of variation explained was reported. B Representative taxonomic distribution (relative abundance) of the sequenced virome. Top pie chart shows the the percentage of the classified, unclassified and unknown at the taxa of family level. The bottom bar chart only includes the classified taxa at the taxonomical level of the family. The taxonomy of contigs was determined by querying the viral contigs against a database containing taxon signature genes for virus orthologous group hosted at www.vogdb.org. The unclassified are the contigs that cannot be assigned to any known viral taxonomy at the family level; the unknown is the contigs that are related to “viral dark matter.” C The volcano plot shows differentially abundant vOTUs identified from DESeq2 analysis, displaying those with a fold change of two or greater when comparing SSLR to xGen. Each dot represents a vOTU contig and is colored to indicate significance. Gray, not significant (NS); green, significant by log2 fold change (> 2); blue, significant by p-value (< 0.01); red, significant by log2 fold change and p-value. D Heatmap of the high abundance (RPKM) viral contigs that were highly enriched in SSLR method that does not present in the rest of methods. This figure shows a subset of the data presented in Fig. S9A. Different colors indicate different annotated protein, and directional boxes indicate open reading frames (ORFs) in the respective orientation. Non-filled arrows indicate no protein hits, and the rest of the colored arrows are hits to the majority of PAU proteins