Fig. 3

Quantification and MDA bias for highly modified T4 genome. A The concentrations of DNAs used in this study were measured by two different quantification methods (Qubit and NanoDrop). B MDA amplification differs in genomes. One and 10 ng of DNA from dsDNA P1, T4, T4-c, and T7 were amplified by 30 min of MDA and purified with Zymol Genomic Purification kit. The y-axis indicates the total yield of amplified MDA products (μg) and measured either by NanoDrop (red) or Qubit (blue). C The MDA amplification dsDNA products (T4, T4-c, P1, and T7) were visualized by TapeStation 4200 with genomic ScreenTape. D Percentage of dsDNA phage genomes with T4 or T4-c generated by different library preparation methods. Different colors indicate different phage genomes with equal input (11.11%). MG, Mock G contains high modification dsDNA T4 genome (T4) with equal ratio of all genomes; MH, Mock H contains lower modification dsDNA T4 genome (T4-c) with equal ratio of all genomes (Fig. S1G)