Fig. 2

Validation of the magnet-based IgA-sorting methods. A Experimental setup. Labeled S. aureus (GFP⁺) is combined with homogenized fecal-derived bacteria before magnetic sorting of IgA⁺ bacteria. B, C Representative dot plots (B) and purity (C) of the pre-sort, IgA⁻ and IgA⁺ fractions using the plate- and column-based formats. PE (IgA) is shown on the Y-axis and GFP (S. aureus and Syto9 (living fecal bacteria)) is shown on the X-axis (B). D Average relative abundance of the bacterial species (> 1%) in the pre-sort and IgA⁺ fractions after plate- and column-based sorting based on 16S rRNA gene sequencing results of three spiked human fecal samples. Bacterial species < 1% are grouped under “Other”. N = 3, experiments are performed in triplicates