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Fig. 4 | Microbiome

Fig. 4

From: Anaerostipes caccae CML199 enhances bone development and counteracts aging-induced bone loss through the butyrate-driven gut–bone axis: the chicken model

Fig. 4

Effect of specific strains or their metabolites on bone metabolism in vitro and in vivo. A Femur length in chicks inoculated with different bacterial strains. B Representative images of femurs obtained by dual-energy X-ray absorptiometry analysis (DEXA). C–E Quantification of DEXA showing the bone volume (BV) (C), bone mineral density (BMD) (D), and bone mineral content (BMC) (E) of the femur. F Measurement of alkaline phosphatase (ALP) activity in the femur. G Measurement of tartrate resistant acid phosphatase (TRAP) activity in the femur. n = 8 per group. H Schematic representation of the experiment in vitro experiment used to investigate the effect of metabolites from different strains on osteogenic mineralization. n = 3 per group. I–K Representative images and quantification of ARS staining in osteoblasts treated with blank GAM (I) or the fermentation products of BC (J) and ANA (K). Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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Microbiome

ISSN: 2049-2618

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