Table 4 Cost estimation for qPCR and ddPCR
From: Highly accurate and sensitive absolute quantification of bacterial strains in human fecal samples
Method | Specifications | Items and time | Cost | Cost per sample |
|---|---|---|---|---|
qPCR | • A standard curve (six serial dilutions, in triplicate) • Twenty-five unknown samples and a no template control (all in triplicate) | Reagents | CAD $60 | CAD $3.2 |
Consumables | CAD $20 | |||
2.5 h | ||||
ddPCR | • A positive control (in triplicate) • Thirty unknown samples and a no template control (all in triplicate) | Reagents | CAD $275 | CAD $12.5 |
Consumables | CAD $100 | |||
6.5 h |