Fig. 3
From: Highly accurate and sensitive absolute quantification of bacterial strains in human fecal samples
The accuracy (represented by the recovery rate) of qPCR and ddPCR. DNA extracted from fecal samples (biological triplicates) spiked with L. reuteri DSM 17938 using QK (A) and PQ (B) were analyzed by qPCR and ddPCR. The recovery rate is defined as the ratio between the cell numbers per gram feces measured and the actual cell numbers spiked, as suggested previously [43]. Results were compared with 100% recovery which is indicated by the dotted line. QK, QIAamp Fast Stool DNA Kit-based method and PQ, protocol Q-based method