Fig. 5
Analysis of oxalate consumption by bacteria. A Oxalate consumption assay was performed without (control) or with 1 × 103, 1 × 104 or 1 × 105 CFU/ml of L. acidophilus or E. coli for 1 h. B Oxalate consumption assay was performed without (control) or with 1 × 103 CFU/ml of L. acidophilus or E. coli for 1, 2 or 3 h. The remaining oxalate concentration in the supernatant was determined by measuring its absorbance at λ214 nm using a UV–visible spectrophotometer compared with a standard curve. Quantitative data were obtained from three independent experiments using independent biological replicates. The error bar represents SD. There were no significant differences observed among groups