Fig. 2
Effect of bacteria on CaOx enlargement. Crystal growth assay was performed without (control) or with 1 × 103 CFU/ml of L. acidophilus or E. coli. A Representative images of CaOx crystals at T0 and T60 that were generated under different conditions. Pseudocolor and overlay views were created to enhance the visualization of differential sizes of crystals at T0 and T60. B Δ crystal size representing the growth of crystals was calculated using Formula II (see Materials and methods) from ≥ 100 crystals in ≥ 10 random fields in each sample. Quantitative data were obtained from three independent experiments using independent biological replicates. The error bar represents SD, and only significant P values are indicated