Table 1 Comparison of Sipros 3, Sipros 4, Calisp, and MetaproSIP on E. coli standard samples
Expected 13C atom% | 1.07% 13C | 2% 13C | 5% 13C | 25% 13C | 50% 13C | 99% 13C | ||
|---|---|---|---|---|---|---|---|---|
Peptidesc | Median of 13C atom% | Sipros 4 | 1.1% | 2.0% | 5.0% | 25.0% | 50.0% | 99.0% |
Sipros 3 | 1.0% | 1.0% | 1.0% | 25.0% | 51.0% | 99.0% | ||
Calisp | 1.1% | 2.0% | 4.9% | NAa | NA | NA | ||
MetaProSIP | 0.8% | 1.2% | 5.3% | 10.2% | 11.8% | 97.7% | ||
MADb of 13C atom% | Sipros 4 | 0.0% | 0.0% | 1.5% | 1.5% | 3.0% | 0.0% | |
Sipros 3 | 0.0% | 0.0% | 0.0% | 4.4% | 5.2% | 0.0% | ||
Calisp | 1.2% | 1.3% | 1.5% | NA | NA | NA | ||
MetaProSIP | 0.3% | 0.1% | 2.7% | 5.7% | 7.6% | 1.0% | ||
Count | Sipros 4 | 18,147 | 14,523 | 11,297 | 8188 | 7275 | 9901 | |
Sipros 3 | 18,003 | 14,824 | 12,420 | 5478 | 2218 | 10,042 | ||
Calisp | 5259 | 6936 | 6137 | NA | NA | NA | ||
MetaProSIP | 11,695 | 10,131 | 5,741 | 1003 | 730 | 85 | ||
Proteins/protein groupsc | Median of 13C atom% | Sipros 4 | 1.1% | 2.0% | 5.0% | 25.0% | 50.0% | 99.0% |
Sipros 3 | 1.0% | 1.0% | 1.0% | 25.0% | 51.0% | 99.0% | ||
Calisp | 1.1% | 2.0% | 4.9% | NA | NA | NA | ||
MetaProSIP | 0.8% | 1.1% | 2.1% | 9.8% | 11.2% | 97.7% | ||
MADb of 13C atom% | Sipros 4 | 0.0% | 0.0% | 0.0% | 1.5% | 1.5% | 0.0% | |
Sipros 3 | 0.0% | 0.0% | 0.0% | 1.5% | 3.0% | 0.0% | ||
Calisp | 1.2% | 1.3% | 1.5% | NA | NA | NA | ||
MetaProSIP | 0.1% | 0.1% | 1.5% | 4.7% | 6.2% | 0.9% | ||
Count | Sipros 4 | 1815 | 1458 | 1210 | 973 | 893 | 1490 | |
Sipros 3 | 1834 | 1546 | 1428 | 871 | 476 | 1491 | ||
Calisp | 723 | 1105 | 973 | NA | NA | NA | ||
MetaProSIP | 1592 | 1429 | 1202 | 549 | 466 | 59 | ||