Skip to main content
Fig. 1 | Microbiome

Fig. 1

From: Proteomic stable isotope probing with an upgraded Sipros algorithm for improved identification and quantification of isotopically labeled proteins

Fig. 1

MS/MS measurement of a peptide’s unlabeled isotopologue with 1.07 atom% 13C and its labeled isotopologue with 50 atom% 13C. The MS1 scans (two upper panels) and the MS2 scans (two lower panels) are shown for the unlabeled isotopologue (two left panels) and the 50% 13C-labeled isotopologue (two right panels) of the peptide GITINTSHVEYDTPTR. Each panel shows both the observed spectrum (upper half) and the theoretical spectrum (lower half). The 13C labeling increased the m/z values and widened the isotopic envelopes of both the precursor ions in the MS1 scans and the product ions in the MS2 scans. The fragmentation pattern of the peptide was similar between its two isotopologues

Back to article page

Microbiome

ISSN: 2049-2618

Contact us