Fig. 6

A combination of FISH, FIB-SEM, and NanoSIMS analyses. a–c LHC-1 cells were mixed with E. coli cells and incubated with NaH¹³CO₃ for 1 h and then fixed and dried on a silicon wafer. Thereafter, the FISH experiment was conducted using a universal bacterial probe EUB338 (a) and an LHC-1-specific probe BTC19 (b), and the bacteria were imaged with an Olympus Optical BX51 fluorescence microscope. c A merged image of a and b. The white arrowheads point to E. coli cells in a–c. d SEM image of the same region of interest (ROI) of a to c, which was acquired at an accelerating voltage of 5 kV with a 6-mm working distance. The LHC-1 cells can be easily distinguished from E. coli due to their disparity in sizes, and the correspondence with the LHC-1-specific probe BTC19 in b. e Pt deposition (yellow arrowheads) on the ROI with FIB-SEM as markers for NanoSIMS imaging. The ROI analyzed using NanoSIMS is outlined as white rectangular. f NanoSIMS image of ¹²C⁻ of the same ROI