Fig. 6

Evolutionary history of syntrophic function in Atribacterota. The Bayesian tree topology was determined by MrBayes [43] by using multiple sequence alignments of 16 ribosomal proteins. The results of the standard deviation of split frequencies (<Â 0.01), the potential scale reduction factor (PSRFÂ =Â 1), and the effective sample size (ESSÂ >Â 100) made the Bayesian tree highly reliable. The time-calibrating phylogenetic tree was further inferred by using R with the ape package. The evolutionary history of Atribacterota was inferred by using COUNT [50] as previously described [29, 51], and further confirmed by phylogenetic trees of each protein set