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Fig. 5 | Microbiome

Fig. 5

From: Cultivation of novel Atribacterota from oil well provides new insight into their diversity, ecology, and evolution in anoxic, carbon-rich environments

Fig. 5

Phylogenetic tree based on concatenated AcsDABCE sequences. Maximum likelihood phylogeny of concatenated AcsDABCE sequences was performed as previous studies [29, 42]. The subunits of AcsD, AcsA, AcsB, AcsC, and AcsE were aligned using MUSCLE with 100 iterations [39], respectively. The alignments were concatenated by using a perl script (https://github.com/nylander/catfasta2phyml). IQ-Tree [41] was used for phylogenetic inference with parameters (-alrt 1000 -bb 1000 -nt AUTO). The best-fit model was LG+F+R8, which was well supported by the Akaike Information Criterion (AIC), Corrected Akaike Information Criterion (cAIC) and Bayesian Information Criterion (BIC). Phylogenetic tree was visualized and annotated using iTOL [66]

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