Fig. 3

Challenges, considerations, and limitations for conducting integrative multi-omics experiments on the endohyphal microbiome. The overall multi-omics workflow consists of sample harvesting, sample extraction, and sample preparation, as well as data generation, data analysis, and data integration steps. The blue arrow denotes progression through the experimental process (from sample isolation to integration of multi-omics data), and the orange triangle denotes the decrease in sample yield as the workflow progresses from sample harvesting and extraction to sample preparation. Created with BioRender.com; adapted from “Multi-Panel Horizontal Timeline (Layout 2 × 2),” by BioRender.com (2023). Retrieved from https://app.biorender.com/biorender-templates