Fig. 6

Polysaccharide utilization potential of M. profundi MTRN7. A Phylogenetic inference of MTRN7 based on 120 conserved single-copy genes identified by GTDB-Tk v1.7.0 [44]. Strain MTRN7 is marked in red while recovered MAGs are marked in blue. TEM observation of MTRN7 was shown. B Predicted PULs in Mesoflavibacter MAGs. C Growth assays of MTRN7 and two reference strains (M. zeaxanthinifaciens DSM 18436 and M. sabulilitoris KCTC 42117) in marine mineral medium (MMM) supplemented with glucose, arabinose, and arabinan at atmospheric pressure. MMM without supplemented sugars or polysaccharides was used as a control. D All genes significantly upregulated (|log2FC| > 1 and FDR-adjusted p < 0.05) when MTRN7 was grown with basal medium plus arabinan compared to basal medium and basal medium plus glucose. Several genes located in the arabinan PUL were further examined by RT-qPCR. Gray bricks indicate genes that were not quantified