Fig. 4

Effect of copper ion dosing on total bacteria and L. pneumophila. Total bacteria (16S rRNA gene copy numbers—circles) and L. pneumophila (mip gene copy numbers—triangles or CFU—diamonds) were measured in SWHs with PEX pipe at 32 °C. No AOC was supplemented. An increasing amount of Cu2+ was dosed over the course of the experiment, from 5 to 1000 ppb as shown in the top panel, to the SWHs indicated in red. Standard errors were determined from triplicate SWHs. Asterisk indicates significant difference in comparison to the no Cu2+ control