Table 1 List of used media and conditions
Medium | Phase | pH | Incubation temperature | Gasphase | Abbreviation | Target organisms | Medium reference |
|---|---|---|---|---|---|---|---|
R2A agar pH 5 | Solid | 5 | 30 °C | Aerobic (ambient) | pH 5 | Acidophiles | – |
R2A agar pH 9 | Solid | 9 | 30 °C | Aerobic (ambient) | pH 9 | Alkaliphiles | – |
R2A agar pH 7 | Solid | 7 | 30 °C | Aerobic (ambient) | pH 7F | Heterotrophs | – |
RAVAN pH 7 for oligotrophs | Solid | 7 | 30 °C | Aerobic (ambient) | RAV | Oligotrophs | [72] (modifieda) |
DSMZ_Medium97 for halophiles | Liquid | 7.5 | 40 °C | Aerobic (ambient) | Halo | Halophiles | DSM 97 (www.dsmz.de) |
R2A pH 7 liquid | Liquid | 7 | 30 °C | N2 | pH 7an | Anaerobes | – |
Medium for methanogens | Liquid | 7 | 40 °C | H2CO2 (80:20) | MS | Methanogens | [73] |
MS supplemented with 0.1% yeast extract and 0.1% acetate | Liquid | 7 | 40 °C | H2CO2 (80:20) | MS_sup | Methanogens | – |
Archaea-supporting liquid medium | Liquid | 7 | 30 °C | N2 | ASM | Archaea | [74]b |
ASM supplemented with 0.1% yeast extract and 0.1% acetate | Liquid | 7 | 30 °C | N2 | ASM_sup | Archaea | – |
Autotrophic all-rounder liquid medium | Liquid | 7 | 30 °C | N2CO2 (80:20) | AAM | Autotrophs | [74]b |
Autotrophic homoacetogen liquid medium | Liquid | 7.5 | 30 °C | H2CO2 (80:20) | AHM | Autotrophs | [74]b |